Ameliorate Effect of Ajwa (Phoenix dactylifera) on C-Reactive Protein, Lipid Profile and Cardiac Histopathology in Rabbits


  • Aisha Saleem M.Phil Researcher School of Zoology, Minhaj University Lahore
  • Irum Naureen Assistant professor, School of Zoology Minhaj University Lahore
  • Aadab Akhtar GC Women university Sialkot
  • Gulnaz Tasleem M.Phil Researcher School of Zoology, Minhaj University Lahore
  • Sara Chaudhry M.Phil Researcher School of Zoology, Minhaj University Lahore
  • Attique Nawaz M.Phil Researcher School of Zoology, Minhaj University Lahore
  • Sabeen Ijaz Centre of Applied molecular Biology and Forensic sciences
  • Muhammad Naeem Institute of Research and Information Mirpur AJK, Pakistan
  • Zaib ul Nisa M.Phil Researcher School of Zoology, Minhaj University Lahore


Cardiovascular diseases, Lipid profile, hs-CRP, CRP, ELISA


Background: High fat diet causes oxidation of accumulated fat cells which leads to Atherosclerosis. The inflammatory marker C-reactive protein has recently been identified as an independent predictor of future coronary heart disease. The inflammatory marker C-reactive protein has recently been identified as an independent predictor of future coronary heart disease. Present study evaluated the cardioprotective role of Ajwa seed paste on lipid profile and C-reactive protein (CRP) level in rabbits. Methodology: 7 months old rabbits (n=15) for 30 days were kept in animal house school under standard laboratory conditions. Rabbits were divided into three groups (n=5). Group1 served as control group fed basal diet, group 2 fed high fat diet, group 3 fed high fat diet supplemented with 6gm ajwa seed paste. Feed consumption ratio was determined in all groups on daily basis.  Results: Feed consumption ratio showed significant (P>0.05) decrease in group 2and 3 fed high fat diet. In this study, lipid profile (cholesterol, triglyceride, LDL and VLDL) values were increased in group 2 compared to control group. It was found that significant (P>0.05) decrease in the level of bad cholesterol (LDL, VLDL) in group 3 (fed ajwa seed supplemented diet).HDL value showed significant (P>0.05) increase in group 3. HDL is good cholesterol and increased the level of HDL protect against the stroke and heart attack. and increase the level of good cholesterol (HDL).HDL takes the cholesterol from the body and back to liver where liver removes the bad cholesterol. The value of CRP is higher in group 2 due to the consumption of HFD and CRP level in group 3 showed significant (P>0.05) reduction.  Conclusion: It is concluded from present study that ajwa seed paste is beneficial for heart health.


Sanchis-Gomar, F. Perez-Quilis, C. Leischik, R. & Lucia, A. (2016) ‘‘Epidemiology of coronary heart disease and acute coronary syndrome’’ Annals of translational medicine, 4(13): 72-80. doi: 10.21037/atm.2016.06.33

Zhang, L. Olalere, D. Mayrhofer, T. Bittner, D.O. Emami, H. Meyersohn, N. M. & Lu, M.T. (2022) ‘‘Differences in cardiovascular risk, coronary artery disease, and cardiac events between Black and White individuals enrolled in the PROMISE Trial,’’ JAMA cardiology, 7(3): 259-267. 10.1001/jamacardio.2021.5340.

Vayalil, P.K. (2012) ‘‘Date fruits (Phoenix dactylifera Linn): an emerging medicinal food,’’ Critical Review. Food Science Nutr., 52(3): 249-271. 10.1080/1 040839 8.20 10.499824

Elsharawy, N.T. AL-Mutarrafi, M. & Al-Ayafi, A. (2019) ‘‘Different types of dates in Saudi Arabia and its most fungal spoilage and its most preservation methods,’’ International Journal of Recent Scientific Research, 10(11):35787-35799, /ij rsr.2019.1011. 1011.4172

Salah, E.B.S.B. El Arem, A. Louedi, M. Saoudi, M. Elfeki, A. & Zakhama, A.L. (2012) ‘‘Antioxidantrich date palm fruit extract inhibits oxidative stress and nephrotoxicity induced by dimethoate in rat,’’ Journal of physiology and Biochemistry, 68(1): 47-58. 10.1007/s13105-011-0118-y

Al-Yahya, M. Raish, M. AlSaid, M.S. Ahmad, A. Mothana, R. A. Al-Sohaibani, & Rafatullah, S. (2016). ‘‘Ajwa?dates (Phoenix dactylifera L.) extract ameliorates isoproterenol-induced cardiomyopathy through downregulation of oxidative, inflammatory and apoptotic molecules in rodent model,’’ Phytomedicine, 23(11):1240-1248. 10.1016 /j.phy med. 2015.10.019

Khalid, S. Khalid, N. Khan, R.S. Ahmed, H. & Ahmad, A. (2017) ‘‘A review on chemistry and pharmacology of Ajwa date fruit and pit,’’ Trends in food science & technology, 6(3): 60-69.

Ahmed Ragab, R. Mohamed Elkablawy, A. Basem Hany, Sheik, Y. & Baraka, N. (2013) ‘‘Antioxidant and Tissue-Protective Studies on Ajwa Extract: Dates from Al-Madinah Al-Monwarah, Saudia Arabia’’ J Environ Anal Toxicol European Journal of Inflammation, 3(1): 163-170.

Hamad, I. AbdElgawad, H. Jaouni, S.A. Zinta, G. Asard, H. Hassan, S. (2015) ‘‘Metabolic Analysis of Various Date Palm Fruit (Phoenix dactyliferaL’’ Cultivars from Saudi Arabia to Assess Their Nutritional Quality, Molecules International Journal of Molecular Sciences, 20(08): 13620-13641. https:// 10.3390/molecules200813620

Hassan, S. M. A. Aboonq, M. S. Albadawi, E. A. Y. Aljehani, H. Abdel-Latif, M. Mariah, R. A. & Elbastawisy, Y. M. (2022) ‘‘The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study: Drug Design, Development and Therapy,’’ International Journal of Molecular Sciences, 1(6): 260-280. 10.2147/DDDT.S344247.

Castell, J. V. Gómez?lechón, M. J. David, M. Fabra, R. Trullenque, R. & Heinrich, P. C. (2019) ‘‘Acute?phase response of human hepatocytes: regulation of acute?phase protein synthesis by interleukin?6 Hepatology, ’’International Food Research Journal, 12(5): 1179-1186. 10.1002/hep.1840120517

Eid, N. M. Al-Awadi, B. Vauzour, D. Oruna-Concha, M. J. & Spencer, J. P. (2013) ‘‘Effect of cultivar type and ripening on the polyphenol content of date palm fruit.’’ Journal of agricultural and food chemistry, 61(10): 2453-2460.

Nehring, S.M. Goyal, A. Bansal, P. & Patel, B. C. (2017) ‘‘C reactive protein,’’ International Journal of Molecular Sciences. 22(23): 130-132. 2 1417/IJN.S121956

Cozlea, D. L. Farcas, D. M. Nagy, A. Keresztesi, A. A. Tifrea, R. Cozlea, L. & Cara?ca, E. (2013) ‘‘The impact of C reactive protein on global cardiovascular risk on patients with coronary artery disease.’’ Current health sciences journal, 39(4): 225-226.

Waqar, A. B. Koike, T. Yu, Y. Inoue, T. Aoki, T. Liu, E. & Fan, J. (2020) ‘‘High-fat diet without excess calories induces metabolic disorders and enhances atherosclerosis in rabbits,’’ Physiological reviews, 213(1): 148-155. /j.atherosclerosis .2010.07.051

Dhungel, S. Sinha, R. Sinha, M. Paudel, B. H. Bhattacharya, N. &. Mandal, M. B. (2019) ‘‘High fat diet induces obesity in British Angora Rabbit: A model for experimental obesity’’ Indian J Physiol Pharmacol, 53(1): 55-60. PMID: 19810577.

Boateng, L. Ansong, R. Owusu, W. B. & Steiner-Asiedu, M. (2016) ‘‘Coconut oil and palm oil's role in nutrition, health and national development: A review. Ghana medical journal, 50(3):189–196. PMCID: PMC5044790

Ahmed, N. Farooq, J. Meo, A. & Pashae, F. (2022) ‘‘Role of Phoenix dactylifera (Ajwa) on lipid profile; a randomized controlled trial,’’ Journal of King Saud University- Science, 34(4): 959-101.

Khalid, S., Arshad, M., Saad, B., Imran, M., Ul Ain, H. B., Tufail, T., & Ahmed, S. (2020). Cardio protective effects of dates. International Journal of Biosciences, 16(5), 110-123. ISSN: 2220-6655.

Jubayer, F. S. Kayshar, & Rahaman, M. (2020) ‘‘Effects of Ajwa date seed powder on serum lipids in humans: A randomized, double-blind, placebo-controlled clinical trial’’. Journal of Herbal Medicine, 2(4): 100-409.DOI:10.1016/j.hermed.2020.100409.

Lee, G.H. Kang, K.K. Yun, H.H. Jo, W. & Oh, T. (2023) ‘‘Multiple time-dependent pathophysiological changes in a rabbit model of high-fat diet-induced hyperlipidemia.’’ FEBS open biology, 13(6): 1027–1040.

Mushtaq, Z., Kausar, S., Kousar, N., & Chiragh, S. (2017). Effect Of Ajwa Date Seed On Lipid Profile Of Diet Induced Hyperlipidemic Rabbits. Khyber Medical University Journal, 9(3), 135–139. Retrieved from

Guo, Z. Ali, Q. Abaidullah, M. Gao, Z. Diao, X. Liu, B. Wang, Z. Zhu, X. Cui, Y. Li, D. & Shi, Y. (2022) ‘‘High fat diet-induced hyperlipidemia and tissue steatosis in rabbits through modulating ileal microbiota’’. Applied microbiology and biotechnology, 106(21): 7187–7207.




How to Cite

Aisha Saleem, Irum Naureen, Aadab Akhtar, Gulnaz Tasleem, Sara Chaudhry, Attique Nawaz, Sabeen Ijaz, Muhammad Naeem, & Zaib ul Nisa. (2024). Ameliorate Effect of Ajwa (Phoenix dactylifera) on C-Reactive Protein, Lipid Profile and Cardiac Histopathology in Rabbits. American Scientific Research Journal for Engineering, Technology, and Sciences, 98(1), 144–157. Retrieved from