Characterization and some Bioactivities of the Synthesized Citrus Pectin-ZnO Nanocomposites from Citron and Pomelo Fruits Peels

  • Dr. Thida Hlaing lecturer, Department of Chemistry, Myitkyina University, Myitkyina, Myanmar
  • Dr. Htin Zaw Myint Associate Professor, Department of Chemistry, Taungoo University, Taungoo, Myanmar
  • Dr. Nwet Nwet Win Associate Professor, Department of Chemistry,University of Yangon, Yangon, Myanmar
  • Dr. Daw Hla Ngwe Professor and head, Department of Chemistry,University of Yangon, Yangon, Myanmar
Keywords: extracted citrus pectins, alcohol precipitation method, pectin- ZnO nanocomposites, co-precipitation method, microbial inhibition, tumor inhibition.

Abstract

Pectin was extracted from the peels of citrus fruits (Citron: Citrus medica L. and Pomelo: Citrus maxima Merr.). In the extraction of fresh and dry pectin, acidic hydrolysis of the fresh or dry fruit peel samples was carried out followed by precipitation with ethanol. The yield percents of extracted pectins were 4.53 % (based on fresh peel) and 21.41 % (based on dried peel) from citron peels, and 3.03 % (based on fresh peel) and 9.18 % (based on dried peel) from pomelo peels. Extracted pectins were characterized by XRD, SEM, FT IR and TG-DTA analysis. The citrus pectin–ZnO nanocomposites were prepared by using co-precipitation method. Citron peel pectin-ZnO (CPPT-ZnO) nanocomposite (90.25 % yield) and pomelo peel pectin-ZnO (PPPT-ZnO) nanocomposite (64.95 % yield ) were prepared by using zinc nitrate and 0.2 M sodium hydroxide solution at 28 ± 0.5 oC. The stirring time require for CPPT-ZnO was found to be 1.5 h and that required for PPPT-ZnO was 2h.  The characteristics of the prepared citrus pectin-ZnO nanocomposites were studied by XRD, SEM, FT IR, TG-DTA, AAS and ED XRF (with C-H balance) spectroscopic methods.  The crystallite sizes of CPPT-ZnO and PPPT-ZnO were 32.30 nm and 24.46 nm determined by XRD analysis.The morphological observation of the SEM results revealed that the sizes of ZnO in CPPT-ZnO and PPPT-ZnO were 70.59 nm and 61.55 nm, and were embedded in the pectin matrix. AAS analyses showed that the zinc ion concentrations in CPPT-ZnO and PPPT-ZnO prepared at 28 ± 0.5 oC were 3.88 × 105 ppm and 5.27 × 105 ppm. Both of the tested samples (CPPT-ZnO and PPPT-ZnO) were observed to show antimicrobial activity with inhibition zone diameters ranged between 15 mm to 20 mm against two tested microorganisms such as Bacillus substilis and Staphylococcus aureus and only CPPT-ZnO aginsted Escherichia coli with inhibition zone diameters of 12 mm. Although both nanocomposites were active in tumor inhibitions, only the CPPT-ZnO was taken as positive in tumor inhibitions which shows inhibition percents 37.09 % (» 20%).

References

[1] P.B. Pashpalatha. “Characterization of Pectin Extracted from Different Fruit Wastes.” Journal of Tropical Agriculture, vol. 40, pp. 53 – 55, 2002.
[2] K.K.Woo. Y.Y. Chang. S.K. Li Hrong and P.Y. Tang. “Pectin Extraction and Characterization from Red Dragon Fruit. (Hylocereus polyrhizus): A Preliminary Study.” Journal of Biological Sciences, vol. 10, pp. 631 – 636, Oct. 2010.
[3] L.Shi. and S. Gunasekaran. “Preparation of Pectin-ZnO Nanocomposite.” Nanoscale Res. Lett , vol. 3 (12), pp. 491-495, Dec. 2008.
[4] C.P. Khare. Citrus medica Linn. and Citrus maxima Merr. Indian Medicinal Plant (Dictionary), pp.155 – 156, 2007.
[5] Htin Zaw Myint. “Production and Some Utilization of Pectin from Tamarind Seeds (Tamarindus indica Linn.) and Pomelo Peel (Citrus grandis Osbeck).” PhD (Dissertation), Yangon: Department of Chemistry, University of Yangon, Myanmar, pp. 29 – 35, 46-47, 50, 2007.
[6] A.U. Rahman. M.I Choudhary. and N.J. Thomsen. “Crown Gall Tumor Inhibition Assay and Antimicrobial Assays” Bioassay Techniques for Drug Development, e. Library, vol.10, Singapore: Taylor & Francis, 2005, PP.10-12, 14-16.
[7] N.R. Ferrigni. N.R. J.E. Putnan. B. Anderson. L.B. Jacobsen. D.E. Nichols. D.S. Moore and J. L. Mellaughlin. “Modification and Evaluation of the Potato Disc Assay and Antitumor Screening of Euphorbiaceae Seeds.” J. Nat Prod., vol.45 (6), pp.679-686, 1982.
[8] A. Muhammad. U. Atika. A. Imtiaz. H. Khizar. and N. Samina..“In vitro Evaluation of Biological Activities of Citrus Leaf Extract.” Sains Malaysiana, vol.43 (2), pp.185-194, May 2014.
[9] P. Srivastava, and R. Malviya. “Source of Pectin, Extraction and Its Application in Pharmaceutical Industry-An Overview.” Indian Journal of Natural Products and Resources, vol. 2 (1), pp.10 -18, Jan. 2011.
[10] M.H. Norziah. E.O. Fang. and A.A. Karim. “Extraction and Characterization of Pectin from Pomelo Fruit Peels.” Gums and Stabilizer for the Food Industry, vol.10, pp.29 – 31, 2000.
[11] R.M. Silverstein. G.C.Bassler and T.C. Morril. “ Organic Chemistry : Chapter. 3” Spectroscopic Identification of Organic Compounds, 7th ed ., New York: Wiley, 1991, pp.71 – 143.
[12] O.Kammori. N. Yamagachi. and K. Sato. “The Infrared Absorption Spectra of Metal Oxides.” Japan Analyst, vol.16 (10), pp.1050-1055, 1967, 1968.
[13] T. Theivasanthi. and Alagar, M. Electrolytic Synthesis and Characterizations of Silver Nanopowder. India: Center for Research and Post Graduate Department of Physics, Ayya Nadar Janaki Ammal College, pp.1-12, 2012.
Published
2017-01-19
Section
Articles