Comparison of Antioxidant and Anti-hyaluronidase Activity of Tomato (Solanum lycopersicum L.) Extract and Lycopene


  • Djohan Djohan Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
  • Kristina Kristina Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
  • Alhoi Hendry Henderson Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
  • I Nyoman Ehrich Lister Department of Physiology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
  • Ermi Girsang Faculty of Public Health, Universitas Prima Indonesia, Medan, Indonesia
  • Edy Fachrial Laboratory of Biomolecular, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia


Antioxidant, Hyaluronidase, Solanum lycopersicum L., Lycopene, Antiaging.


Aging process contributed with poor of lifestyle and photodamage can induced wrinkles formation because of extracellular matrix destruction. To investigate the potential of tomato as antiaging sources particularly as the hyaluronidase inhibitor. This study used H2O2 scavenging activity for antioxidant assay and hyaluronidase inhibition activity for the antiaging property.  The Solanum lycopersicum L. extract (SLE) has lower antioxidant activity (221.30 ± 1.94) compared with lycopene (208.37 ± 4.87) through H2O2 scavenging activity. The Solanum lycopersicum L. (119.81 ± 14.23) has lower antiaging activity particularly as anti-hyaluronidase activity compared with lycopene (81.65 ± 5.95). Our findings suggest that the Solanum lycopersicum L. has weak antioxidant activity and moderate antiaging activity, particularly as a hyaluronidase inhibitor. Solanum lycopersicum L. can be used as an antiaging source, particularly as hyaluronidase inhibitor.


Q. Wang, F. Zhou, W. Xie, X. Zhao, X. Liu. “Research Progress on Aging Mechanisms”. Advances in Aging Research, vol 5, pp. 49-57. Mar. 2016.

K. Renton, KY. Keefe. “Treating and Preventing Tissue Shift and Wrinkles Caused by Compression”. Journal of Aesthetic Nursing, vol 4(2), pp. 8-11. Mar. 2015.

K. Naidoo, MA. Birch-Machin. “Oxidative Stress and Ageing: The Influence of Environmental Pollution, Sunlight and Diet on Skin”. Cosmetics, vol. 4(4), pp. 1-8. Jan. 2017

F. Poon, SKL. Chien. “Mechanisms and Treatments of Photoaging”. Photodermatology Photoimmunology Photomedicine, vol. 31, pp. 65-74. Oct. 2015.

T. Shahzad, I. Ahmad, S. Choudhry, MK. Saeed, MN. Khan. “DPPH Free Radical Scavenging Activity of Tomato, Cherry Tomato and Watermelon: Lycopene Extraction, Purification and Quantification”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6(2), pp. 223-8. Jan. 2014.

A. Elias, SA. Mutalib, WAW Mustapha, S. Shahimi, N. Mohamed, RAM. Repin. “Antioxidant content of tomatoes (Lycopersicon esculentum cv MT1) treated by different type of pesticide, fertilizer and growth medium in compost”. International Journal of ChemTech Research, 11(5), pp. 387-93. 2018.

PM. Murray, S. Moane, C. Collins, T. Beletskaya, OP. Thomas, AWF. Duarte, FS Nobre, IO Owoyemi, et al. “Sustainable Production of Biologically Active Molecules of Marine Based Origin”. New Biotechnology, vol. 30(6), pp. 839-50. Sep. 2013.

E. Papakonstantinou, M. Roth, G. Karakiulakis. “Hyaluronic Acid: A Key Molecule in Skin Aging”. Dermato-Endocrinology, vol. 4(3), pp. 253-8. Dec. 2012.

A. Wiem, A. Smail, M. Wissem, M. Faleiro, M. Miguel. “Antioxidant, Anti-inflammatory and Anti-acetylcholinesterase Activities of Leaf, Flower and Seed Aqueous Extracts of Lawsonia inermis From Tunisia”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6(5), pp. 445-52. May. 2014.

SH. Nile, YS. Keum, AS. Nile, SS. Jalde, RV. Patel. “Antioxidant, Anti-inflammatory, and Enzyme Inhibitory Activity of Natural Plants Flavonoids and Their Synthesized Derivates”. J. Biochem. Mol. Toxicol, vol. 32, pp. 1-8. Sep. 2018.

W. Widowati, N. Fauziah, H. Herdiman, M. Afni, E. Afifah, HSW. Kusuma, H. Nufus, S. Arumwardana, DD. Rihibiha. “Antioxidant and Anti Aging Assays of Oryza Sativa Extracts, Vanillin and Coumaric Acid”. Journal of Natural Remedies, vol. 16(3), pp. 88-99. Jul. 2016.

S. Utami, P. Adityaningsari, I. Sosiawan, S. Endrini, QR. Sachrowardi, SP. Laksono, S. Nafik, BC. Arrahmani, et al. “Antioxidants and Anticholinesterase Activities of the Characterized Ethanolic of ripe Sesoot (Garcinia picrorrhiza Miq.) Fruit Extract (GpKar) and Xanthone”. Traditional Medicine Journal, vol. 22(3), pp. 160-5. Dec. 2017.

W. Widowati, WB. Janeva, S. Nadya, A. Amalia, S. Arumwardana, HSW. Kusuma, Y. Arinta. “Antioxidant and Antiaging Activities of Jasminum Sambac Extract, and Its Compounds”. Journal of Reports in Pharmaceutical Sciences, vol. 7(3), pp. 270-85. Jul. 2018.

YH. Huang, PY. Wu, KC. Wen, CY. Lin, HM. Chiang. “Protective Effects and Mechanisms of Terminalia catappa L. Methanolic Extract on Hydrogen-Peroxide-Induced Oxidative Stress in Human Skin Fibroblasts”. BMC Complementary and Alternative Medicine, vol. 18, pp. 1-9. 2018.

S. Muthu, B. Durairaj. “Evaluation of Antioxidant and Free Radical Scavenging Activity of Annona muricate”. European Journal of Experimental Biology, vol. 5(3), pp. 39-45. 2015.

V. Soundararajan, S. Sreenivasan. “Antioxidant Activity of Elaies guineensis Leaf Extract: An Alternative Nutraceutical Approach in Impeding Aging”. APCBEE Procedia, vol. 2, pp. 153-9. 2012.

R. Sundararajan, R. Ilengesan. In vitro Antioxidant Assay of Methanol Extract of Buddleja asiatica”. Free Radicals Antioxidants, vol. 8(1), pp. 55-61. Jun. 2018.

I. Firdrianny, A. Rizkiya, K. Ruslan. “Antioxidant Activities of Various Fruit Extracts from Three Solanum sp. Using DPPH and ABTS Method and Correlation with Phenolic, Flavonoid and Carotenoid Content”. Journal of Chemical and Pharmaceutical Research, vol. 7(5), pp. 666-72. 2015.

J.Treml, K.Smejkal. “Flavonoid as Potent Scavengers of Hydroxyl Radicals”. Comprehensive Reviews in Food Science and Food Safety, vol. 15, pp. 720-38. 2016.

G. Ndlovu, G. Fouche, M. Tselanyane, W. Cordier, V. Steenkamp. “In Vitro Determination of the Anti-Aging Potential of Four Southern African Medicinal Plants”. BMC Complementary and Alternative Medicine, vol. 13, pp. 1-7. Dec. 2013.

GD. Liyanaarachchi, JKRR. Samarasekera, KRR. Mahanama, KDP. Hemalal. “Tyrosinase, Elastase, Hyaluronidase, Inhibitory and Antioxidant Activity of Sri Lankan Medicinal Plants for Novel Cosmeceuticals”. Industrial Crops and Products, vol. 111, pp. 597-605. Nov. 2018.




How to Cite

Djohan, D., Kristina, K., Hendry Henderson, A., Ehrich Lister, I. N., Girsang, E., & Fachrial, E. (2019). Comparison of Antioxidant and Anti-hyaluronidase Activity of Tomato (Solanum lycopersicum L.) Extract and Lycopene. American Scientific Research Journal for Engineering, Technology, and Sciences, 52(1), 49–56. Retrieved from