The Result of Some Biomarker of Free Radical-Peroxidation of Lipids and Antioxidant System of Experimental Acute Infarction Model Induced by Coronary Artery Occlusion in Rats

Abstract

Background: Recently years, Increasing coronary artery disease, diabetes, and cancer in the worldwide. Epidemiological studies indicate that ischemic heart disease will constitute the major disease-burden worldwide by the year 2020. Myocardial infarction is invariably followed by numerous pathophysiological and biochemical alterations including hyperlipidemia, thrombosis, lipid peroxidation and free radical damage etc., leading to qualitative and quantitative changes of myocardium. Determination of in blood serum and cardiac tissue homogenate levels of cardiac biomarker cardiac specific troponin I and estimation of lipid peroxidation products and some antioxidants enzymes of experimental acute infarction model induced by coronary artery occlusion in rats. Materials and methods: Adult male Wistar rats, weighting approximately 180 to 200±20 gram, used for the experiment. The were divided randomly into 2 groups (6-8 animals in each group). They were distributed as follow: first group (healthy, non treatment), second group (control, experimental acute infarction model induced by coronary artery occlusion in rats,  non treatment). We did coronary occlusion induced myocardial ischemia by Kogan A.K., Ambaga M /1979/‘s method.

The experiment is 1^st, 3^rd, 7^th, 14^th 21^st days blood samples were collected and used to determine in blood serum and cardiac tissue homogenate levels of cardiac biomarker cardiac specific troponin I (CT_n-I) and estimation of lipid peroxidation products (MDA, SA, LPO) and some antioxidants enzymes (SOD, GSH, GSH-px) were estimated using standard rat ELISA KIT by enzyme-linked immunesorbent assay. Result: Determination of cardiac troponin-I levels in blood serum of experimental acute infarction model induced by coronary artery occlusion in rats increased by 31.9-58.4% in the 1^st, 3^rd, 7^th, 14^th 21^st days of the test compared to healthy groups. The cardiac biomarker cardiac specific troponin I is indicative for cardiomyocyte damage and is currently used in the diagnosis and prognosis of myocardial ischemia. Experimental acute infarction model induced by coronary artery occlusion in white rats, it is sufficiently shown that from day 1-21 MDA in plasma increased by 43.9 – 76.3%, plasma SA by 18.3-49.8% and LPO by 12.8-38.3%. This results in necrosis of cells and tissues, dissolving of membrane and stimulation of pathogenesis of disruption of membrane parts. It is also shown that substances such as endogen and antioxidant decreases in heart ischemia. Conclusion: Determination of increased level are lipid peroxidation products (MDA, SA, LPO, CT_n-I), and decreased level are antioxidants (SOD, GSH, GSH-px) in blood serum of experimental acute infarction model induced by coronary artery occlusion in rats, it seems to induced of pathogenesis of coronary disease and infarction myocardium while the accumulation of lipid product cause to damage the cell membrane.