Bacteriological Evaluation of Drinking Water of Rajshahi City, Bangladesh
Keywords:Bacteria, Pollution, drinking water, Rajshahi
Access to pathogen free safe drinking water is one of the major challenges of the 21st century for most of the people of world. According to WHO guidelines, 100 ml sample of safe drinking must be free of total coliform, fecal coliform and E. coli. Hence, this study was designed for bacteriological evaluation of drinking water collected from different restaurant in Rajshahi city, Bangladesh. Among 10 samples, no detectable E. coli was found in 5 water samples (100 ml each) while 8-50 detectable E. coli in 100 ml water sample was found in the remaining 5 samples which exceeded the BDS and WHO standard of drinking water. Four randomly selected isolates (B1, B2, B3 and R) which were isolated from different water samples were subjected to 16S rRNA gene sequencing. It was found that the 16S rRNA sequence of isolate B1, B2 and B3 had 98% similarity to that of Enterobacter asburiae, Escherichia coli and Acinetobacter sp respectively. Similarly, 16S rRNA sequence of isolate R had 99% similarity to that of Klebsiella pneumonia. Results of biochemical analysis revealed that all four isolates were negative to Catalase, KOH and Oxidase test while variations were found for other tests. Similarly, all four isolates were able to ferment all tested carbohydrates except cellulose. The optimum pH and temperature for the growth of the all isolates was pH 7.0 and 37ºC. It was found that all the isolates were sensitive to majority of the antibiotics used.
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