Histological and Immunocytochemical Studies on the Buccal Minor Salivary Glands in Rabbits


  • Ghofran F. AL-Jubuori Laboratory investigation Department, College of Sciences, University of Kufa , Iraq
  • Wafaa H. AL-hashemi Basic Sciences Department, College of Dentistry, University of Kufa, Iraq
  • Akram . Y. Yasear Basic Medical Sciences Department, College of Dentistry, University of Kerbala, Iraq
  • Mohammed E. mansur


ducal gland, minor salivary glands and myoepithelial cells


Buccal minor salivary glands are continuously produced saliva ,which is important to lubricate the buccal mucosa.Buccal glands were collected from thirty adult male rabbits. Wall of cheek were removed from anesthetized rabbits at the place of  buccal glands. Paraffin sections stained of the glands were stained by  H and E stain, PAS reaction and alcian blue (pH 2.5) stain.   Immunocytochemistry to stain actin myofilaments of myoepithelial cell were applied. It’s were mixed glands, but predominantly mucous in rabbit. The serous cells were represented by demilunes capping mucous tubules together with small number of serous acini. The myoepithelial cells (MECs) were seen around the mucous secretory units and around the intercalated ducts and striated ducts .The MECs were positively stained immunohistochemically for actin myofilaments. The cells secreotory mucous acini  estimation of number and diamstter of ducts and thickest of connective tissue as well as number of acini mucous, serous acini and intercalated ducts and describe of the MECs.


. E. Michael, D. Colin, O'Mullane and Denis. ‟Saliva and oral health” (4th ed.). Little Steine, Hill Farm Lane, Duns Tew, OX25 6JH: Stephen Hancocks Limited, 2012. P. 1.

. H. M. Bushaalaa, A. Y. Yasearb, A. A. Sultan, A. A. Khatal. ‟Effect of Pilocarpine on Minor Salivary Glands of Rabbits” : A Histological Study Libyan Journal of Dentistry.3(1), 2019. Pp.1-10

. L. Eliasson, A. Carlen. ‟An update on minor salivary gland secretions”. Eur J Oral Sci.; 118: 2010. Pp. 435–442.

. R. Hand, D. Pathmanathan , R. B. Field . ‟Morphological features of the minor salivary glands”. Arch Oral Biol. 44 Suppl 1: 1999. Pp. 3-10.

. Nanci. ‟Ten Cate's Oral Histology: Development, Structure, and Function”. 8th ed Pheladelphia: Elsevier Mosby. (1): 2013.pp. 290-317.

. T. Ettl, J. Kleinheinz, R. Mehrotra, S. Schwarz , T. E. Reichert and O. Driemel. ‟Head and Face Medicine, 4: 2008. P.16

. M. N. Rizeq, M. VanDe, M. R. Hendrikson and R. V. Rose. ‟A comparative immunohistochemical study of uterine smooth muscle neoplasms with emphasis on the epithelioid variant” Hum. Patho. 25: 1994. Pp. 671-677.

. P. A. Brennan, T. Umar, G. A. Zaki. et al., ‟Are myoepithelial cells responsible for the wide spread expression of inducible nitric oxide synthase in pleomorphic adenoma? An immunohistochemical study” J Oral Patho Med. 29: 2000. Pp. 279-283.

. J. K. Avery. ‟Essential of oral histology and embryology”. 2nd ed., 2002. Pp. 182-193.

. W. H. AL-Hashemi, M. E.Mansur and M. H. Homady. ‟Intracomparative And Immunocytochemistry Study Of Parotid, Submandibular And Sublingual Gland In Domestic Rabbit Of Iraq”. Biochem. Cell. Arch. 19 (1): 2019a. pp. 1069-1090.

. K. Lorenz, M. Bader, A. Klaus, W. Weiss, A. Gorg and HT. Orosensory. ‟Stimulation effects on human saliva proteome”. J Agric Food Chem: 59: 2011. Pp. 10219–10231.

. H. M. Bushaalaa, A. Y. Yasearb, A. A.Sultan, A. A. Khatal. ‟Effect of Pilocarpine on Minor Salivary Glands of Rabbits” : A Histological Study Libyan Journal of Dentistry.3(1): 2019. Pp. 1-10

. K. M Haddao and A. Y. Yasear. ‟Weber’s Salivary Glands Of Rabbit : Histological And Histochemical Studies”. Biochem. Cell. Arch. 18 (1): 2019. Pp. 557-560.

. M. Stoeckelhuber, D. J. Loeffelbei, B. Olzowy, C. Schmitz, S.Koerdt and M. R. Kesting ‟Labial Salivary Glands in Infants: Histochemical Analysis of Cytoskeletal and Antimicrobial Proteins”. Journal of Histochemistry & Cytochemistry : 64(8), 2016. Pp. 502–510

. Ashok, K. Shah, A. F. Mulla and M. Mayank ‟Pathophysiology of myoepithelial cells in salivary glands”. 20 (3): 2016.pp. 480-490.

. R. B. Rossoni, A. B. Machado, C. R. S. Machado. ‟A histochemical study of catecholamines and cholinesterases in the autonomic nerves of the human minor salivary glands”. The Histochemical Journal. Springer, 11(6): 1979. Pp. 661–668.

. R. F. Ianez, M. E. Buim, C. M. Coutinho-Camillo, R. Schultz, F. A. Soares and S. V. Lourenco ‟Human salivary gland morphogenesis: Myoepithelial cell maturation assessed by immunohistochemical markers”. Histopathology 57: 2010. Pp. 410–417.

. W. H. AL-Hashemi and M. E. Mansur ‟Ultrastructure and estimation of element of tissue of each the parotid, submandibular and sublingual gland of domestic rabbit in Iraq”. Sci. & Res. 11(4), 2019b. pp. 1231-1243.

. S. Asakawa, M. Yamamoto, Y. Katori, G. Murakami, M. Kasahara, S. Matsunaga, and S. Abe. ‟Innervation of submandibular and sublingual glands in elderly donated cadavers: a preliminary histological study of differences in nerve morphology between mucous and serous acini”. Anatomy & Cell Biology. Anat Cell Biol. 48 (1): 2015. Pp. 36-43.

. B. Tandler, K . Toyoshima and C. J. Phillips. ‟Ultrastructure of the principal and accessory submandibular glands of the common vampire bat”. Am. J. Anat., 189: 1990. Pp. 303-315.

. B. Tandler ‟Introduction to mammalian salivary glands”. Microsc Res Tech (26): 1993. Pp. 1–4.




How to Cite

F. AL-Jubuori, G. ., H. AL-hashemi, W. ., Y. Yasear, A. ., & E. mansur, M. . (2019). Histological and Immunocytochemical Studies on the Buccal Minor Salivary Glands in Rabbits. American Scientific Research Journal for Engineering, Technology, and Sciences, 59(1), 154–161. Retrieved from https://www.asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/5074