Mini Review: Q Fever (Coxiellosis): Epidemiology, Pathogenesis and Current Laboratory Diagnosis
Keywords:
Coxiella burnetii, Q fever, PCR, EIA IgM/IgG, IFAAbstract
Q fever is zooantroponozis with global distribution caused by the strictly intracellular bacterium Coxiella burnetii. Causative agent of Q fever is an obligate intracellular parasite, classified in the genus Coxiella, family Coxiellaceae, class Gammaproteobacteria. The importance of the disease was assessed both in terms of human health and the serious economic damage they cause on livestock. Clinical manifestation of Q fever in humans is characterized by a wide variety - from asymptomatic infection to a chronic disease that can be fatal. Several basic methods have been developed to detection of C. burnetii. PCR and C. burnetii genomic sequences in whole blood are a sensitive and safe method of detection, with >90% sensitivity. A four-fold or greater rise of (CF) antibody (phase 2) between the paired sera is also diagnostic approach. Sensitivity of a four-fold rise in titre has been estimated as 73% ÷78% and specificity has been estimated as 90%, respectively. EIA is method with highly sensitive and specific. EIA detect IgM and then IgG antibodies which develop to phase II antigens in 10 to 14 days from symptom onset. IFA tests are of particular value for confirmation of acute infection and for diagnosis of chronic infection with high sensitivity. The technique detected IgG, IgM and IgA immunoglobulin classes. Suitable specimens for C. burnetii detection are blood samples. Although scientific interest in Q fever has always existed, a number of facts concerning the unforeseen nature of the epidemic, various clinical manifestations both in humans and in animals, the opportunities for chronic and other features of infection remain unclear. For this reason, timely and highly sensitive laboratory diagnosis is crucial for the outcome of the disease and subsequent treatment and monitoring.
References
. Parker, N., J. Barralet, A. Bell. (2006). “Q fever”. Lancet, 367, 679 – 688.
. Porter S, Czaplicki G, Mainil J, Guatteo R, Saegerman C. (2011). ‘Q Fever: Current State of Knowledge and Perspectives of Research of a Neglected Zoonosis’. Int J Microb, Art. ID 248418, 22 pages
. Ruiz-Fons F, Rodriguez O, Torina A, Naranjo V, Gortazar C, Fuente J. (2008). “Prevalence of Coxiella burnetii infection in wild and farmed ungulates”. Vet Microb, 126, 282 – 286.
. Maurin, M. & D. Raoult. (1999). “Q Fever”. Clin Microb Rev, 12, 518 - 533.
. Kagawa F., Wehner J, Mohindra V. (2003). “Q fever as a biological weapon”. Eur PMC, 18, 183 - 195.
. Fournier P., Marrie T, Raoult D (1998). “Diagnosis of Q fever”. J Clin Microb, 36, 1823 – 1834.
. Angelakis E. & Raoult D. (2010). “Q fever”. Vet Microb, 140, 297 - 309.
. OIE Terrestrial Manual, 2.1.1.6. Chapter Q fever. (2015)
. Sekoyova Z, Roux V, Raoult D. (1999). “Intraspecies diversity of Coxiella burnetii as revealed by com1 and mucZ sequence comparison”. FEMS Microbiol Lett, 180, 61- 67.
. Seshadri R, Paulsen I, Eisen J, Read T, Nelson K, Nelson W, Ward N. et al. (2003). PNAS, 100, 5455 – 5460.
. Glazunova O, Roux V, Freylikman O, et al. (2005) “Coxiella burnetii genotyping”. Emerg Inf Dis J, 11, 1211– 1217.
. Jager C., Willems D, Thiele G. Baljer (2002). “Molecular characterization of Coxiella burnetii isolates”. Epid & Inf, 120, 157 – 164.
. Tilburg J, Rossen J, Hannen E. van, et al. (2012). ” Genotypic diversity of Coxiella burnetii in the 2007–2010. Q fever outbreak episodes in the Netherlands”. J Clin Microbiol, 50, 1076 – 1078.
. Hendrix L, Samuel J, Mallavia L. (1991). ”Differentiation of C. burnetii isolates by analysis of restriction-endonuclease-digested DNA separated by SDS-PAGE”. J Gen Microb, 137, 269–276.
. Valkova, D. & J. Kazar. (1995). “A new plasmid (QpDV) common to Coxiella burnetii isolates associated with acute and chronic Q fever”. FEMS Microbiol Lett, 125, 275 – 280.
. Derrick E. (1937). ”The course of infection with Coxiella burnetii”. Med J Austr, 1, 1051 - 1057.
. EFSA Panel on Animal Health and Welfare, EFSA Panel on Biological Hazards. Scientific Opinion on Q fever. (2010). EFSA Journal, 8, 1595 – 1667.
. Tissot-Dupont H, Raoult D, Brouqui P, et al. (1992). “Epidemiologic features and clinical presentation of acute Q-fever in hospitalized patients – 323 French cases”. Am J Med, 93, 427 - 434.
. Tjwa M, De Hertogh G, Neuville B, et al. (2001). ”Hepatic fibrin-ring granulomas in granulomatous hepatitis: report of four cases and review of the literature”. Acta Clin Belg, 56, 341 – 348.
. Honarmand, H. (2012). “Q fever: an old but still a poorly understood disease”. Inter Persp Inf Dis, Article ID 131932, 8 pages.
. Morguet A, Jansen A, Raoult D, Schneider T. (2007). “Late relapse of Q fever endocarditis”. Clin Res Cardiol, 96, 519 – 524.
. Tissot-Dupont H, Amadei M, Nezri M, Raoult D. (2004). “Wind in November. Q fever in December”. Emerg Infect Dis, 10, 1264 – 1269.
. Scola B. L. (2002). “Current laboratory diagnosis of Q fever. Seminars in pediatric infectious diseases”. 13(4), 257–262. Available: https://doi.org/10.1053/spid.2002.127199
. Bartelink A, Stevens H, van Kregten, E, Meijer J, Beeres M, & van Deuren M. (2000). “Acute en chronische Q-koorts; epidemiologie, symptomen, diagnostiek en therapie van infecties met Coxiella burnetii [Acute and chronic Q fever; epidemiology, symptoms, diagnosis and therapy of infection caused by Coxiella burnetii]”. Nederlands tijdschrift voor geneeskunde, 144(27), 1303–1306.
. https://www.cdc.gov/mmwr/preview/mmwrhtml/rr6203a1.htm
. Graves S, Massung R. (2015). “Coxiella. In: Manual of Clinical Microbiology”. 11th Edition ASM Press.
. Schneeberger P, Hermans M, van Hannen E, Schellekens J, Leenders A, Wever P. (2009, Dec). “Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever”. Clin Vaccine Immunol. 17(2):286-90. Available: https://doi: 10.1128/CVI.00454-09.
. Musso D, Raoult D. (1995). “Coxiella burnetii blood cultures from acute and chronic Q-fever patients”. J. Clin. Microbiol. 33:3129 –3132.
. Dupuis G, Péter O, Peacock M, Burgdorfer W, Haller E. (1985, Oct). “Immunoglobulin responses in acute Q fever”. J Clin Microbiol. 22(4):484-7
. Field P, Mitchell J, Santiago A, Dickeson D, Chan S, Ho D, Murphy A, Cuzzubbo A, Devine P. (2000, Apr). “Comparison of a commercial enzyme-linked immunosorbent assay with immunofluorescence and complement fixation tests for detection of Coxiella burnetii (Q fever) immunoglobulin”. M. J Clin Microbiol. 38(4):1645-7.
. D'Harcourt S, Soto A, Burgos V, Calero D, Martínez-Zapico R. (1996, Sep). “Comparison of immunofluorescence with enzyme immunoassay for detection of Q fever”. Eur J Clin Microbiol Infect Dis. 15(9):749-52.
. Fournier, P. E., Marrie, T. J., & Raoult, D. (1998). Diagnosis of Q fever. Journal of clinical microbiology, 36(7), 1823–1834. https://doi.org/10.1128/JCM.36.7.1823-1834.1998
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