TY - JOUR AU - P, Ingala AU - F, Lepira AU - L, Mputu PY - 2017/12/10 Y2 - 2024/03/29 TI - Validation of Irma Cgea/crenk One Method of Evaluation of the Vitamin D at the Regional Center of Nuclear Study Laboratory of Kinshasa JF - American Scientific Research Journal for Engineering, Technology, and Sciences JA - ASRJETS-Journal VL - 38 IS - 2 SE - Articles DO - UR - https://www.asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/3668 SP - 59-67 AB - <p>The aim of the present study was to validate the method IRMA CGEA/CRENK for the evaluation of the vitamin D status by comparison to the commercial method DIASORIN. A blood sample was obtained from 30 volunteers with a good health state in general population (11 women and 19 men) to determine serum 25(OH) D, concentrations by all those methods. Using local laboratory cutoffs, vitamin D insufficiency and deficiency was defined as 25(OH) D values of 4-14 ng/ml and ? 4 ng/ml, respectively; vitamin D sufficiency as ?15ng/ml [23]. Statistical analyses were performed using Excel 12.0 and SPSS 21 statistical software’s. Mean and standard deviation (SD), were used to evaluate the mean of vitamin D of the two methods. Student t TEST was used to compare mean of the two methods.  P value ? 0.05 defined the level of statistical significance. PEARSON correlation coefficient (<strong>r</strong>) was performed to evaluate the correlation between the two methods.  An r=0 means the missing of correlation: r&lt;0 means a negative correlation; r=1 means a positive and perfect correlation; 0, 75&lt;r&lt;1 means a positive and strong correlation.  The concordance between IRMA CGEA/CRENK and the commercial method DIASORIN has been definite as the missing of statistic difference between the means of vitamin D with the two methods and a positive correlation between the levels of vitamin D of the two methods. The coefficient kappa was calculated for avoiding the rate of random in the concordance. The variations of the coefficient Kappa is ±1.  K= -1 means total discordance.  K=+1 means an absolute concordance. K near 0 means a mean concordance explain by random. K&gt;0, 8 (80%) is the limit of the better concordance. No statistic difference was observed between the means of vitamin D of the method IRMA CGEA/CRENK and the commercial method DIASORIN (<strong>p=0,330</strong>).  The coefficient of correlation was: <strong>r = 0, 96</strong>. The equation of correlation was: <strong>y =1,429 + 1, 02 x</strong> (y= IRMA CGEA/CRENK, x= DIASORIN Commercial).  The coefficient kappa = <strong>0, 85</strong>.  </p> ER -