Determination Sunscreen Potential of Grapeseed Oil (Vitis Vinifera) in Cream Preparation with Combination of Oxybenzone and Octyl Methoxycinammate by in Vitro Method

  • Iis Sundari Master of Biomedical Science, Faculty of Medicine, University of Prima Indonesia, Medan, North Sumatera, Indonesia
  • Chrismis Novalinda Ginting Faculty of Medicine, University of Prima Indonesia, Medan, Indonesia
  • Linda Chiuman Faculty of Medicine, University of Prima Indonesia, Medan, Indonesia
  • I Nyoman Enrich Lister Department of Physiology, Faculty of Medicine, University of Prima Indonesia, Medan, North Sumatera, Indonesia
Keywords: Sunscreen, Grape seed oil, SPF (Sun Protecting Factor), Oxybenzone, Octyl Methoxycinammate


Grapeseed oil is usually used in cosmetic industry, as anti dandruff, anti microba, antioxidant and sunscreen. The main compound of grapeseed oil is flavonoid, such as proantocyanidine and alfa tocopherol which can protect skin from the impact of ultraviolet radiation. The purpose of this study was to evaluate the influence of adding grapeseed oil to increase the effectiveness of sunscreen preparations containing the combination of oxybenzone and octyl methoxycinamate by measuring the SPF (Sun Protecting Factor) value as its parameter. The preparation was made in 5 formulas such as F1 (blank), F2 (2% oxybenzone and 5% octyl methoxycinammate), F3 (1% GSO), F4 (5% GSO), F5 (10% GSO) combine with 2% oxybenzone and 5% octyl methoxycinammate in each formulas. Evaluations conducted on cream homogeneity, pH, and determining the SPF value of the preparations in vitro method by using spectrophotometer UV-Vis. Then, value of SPF will be tested  in One Way Anova and continued with Post Hoc Tukey. The evaluation results showed that all samples were homogen, in range 6.87 – 6.93 pH, and the average SPF value from each formula were: F1 9.23; F2 22.32; F3 26.16; F4 26.95; and F5 27.44. F1 can be used optimally as sunscreen with maximal protection category while F2, F3, F4, F5 were in ultra protection category. From the result of statistic tests, there was a significant difference on the SPF value between F1 and F2, while in F3, F4, and F5 there is no significant difference on value. In conclusion, the addition of grapeseed oil can increase the SPF value but not significantly between each formula combined with oxybenzone and octyl methoxycinammate in cream preparations.


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